Recombinant Mouse 4-1BB/TNFRSF9 Protein (aa 1-211, Fc Tag)(Active)

Recombinant Mouse 4-1BB/TNFRSF9 Protein (aa 1-211, Fc Tag)(Active)

SKU #	PKSM040474
Expression Host	HEK293 Cells

Description

Synonyms	4-1BB,A930040I11Rik,AA408498,AI325004,CDw137,Cd137,ILA,Ly63,Secreted CD137 antigen,Tnfrsf9 ,Tumor necrosis factor receptor superfamily member 9
Species	Mouse
Expression Host	HEK293 Cells
Sequence	Met 1-Leu 211
Accession	NP_001070976.1
Calculated Molecular Weight	47.0 kDa
Tag	C-hFc
Bio-activity	Immobilized mouse His-TNFSF9 at 10 g/ml (100 l/well) can bind mouse TNFRSF9-Fc, The EC50 of mouse TNFRSF9-Fc is 12.0-29.0 ng/ml.

Properties

Purity	> 90 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptorassociated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.